The assisted hatching is a micromanipulation technique performed usually on the 3-6 day-old embryo, closely before its introduction into the uterus. The erosion of the zona pellucida of the embryo makes its migration and later nesting easier.
The AH can be performed mechanically (with a glass micromanipulation awl), chemically (acid Thyrode solution), with a laser or using an enzymatic method.
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